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KMID : 0369820090390010059
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Jorunal of Korean Pharmaceutical Sciences
2009 Volume.39 No. 1 p.59 ~ p.63
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Effect of Tripolyphosphate (TPP) on the Controlled Release of Cyclosporin A from Chitosan-coated Lipid Microparticles
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Cheon Ji-Woong
Shim Chang-Koo
Chung Suk-Jaeng
Kim Dae-Duk
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Abstract
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Soybean phosphatidylcholine microparticles loaded with cyclosporin A (CsA) were prepared by the modified emulsion solvent diffusion and ionic gelation method, in which chitosan on the surface of the microparticles was crosslinked with various concentrations of tripolyphosphate (TPP). The morphology of the particles was characterized by scanning electron microscopy (SEM). The change of particle size and zeta-potential by chitosan on the surface of the lipid microparticles were systematically observed. The encapsulation efficiency and loading capacity of CsA in the particles were determined by high performance liquid chromatography (HPLC). In vitro release kinetics was studied using the dialysis method. In the results, the mean particle size and the zeta-potential of lipid microparticles increased when the attached chitosan was cross-linked (from 2.5 to 6.2 and from -37.0 to +93.0 mV, respectively). The cyclosporin A-loaded lipid microparticles appeared discrete and spherical particles with smooth surfaces. The encapsulation efficiency of CsA was between 79% and 90% while the loading capacity was between 41% and 56%. In vitro release study showed that the crosslinkage of chitosan by TPP significantly delayed the release of CsA from the particles in a concentration-dependent manner. Thus, the release of CsA from the lipid microparticles could be controlled by tripolyphosphate used as a cross-linking agent.
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KEYWORD
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Cyelosporin A, Chitosan, Tripolyphosphate, TPP, Microparticles, Controlled release
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